lncRNA H19 Promotes Ox-LDL-Induced Dysfunction of Human Aortic Endothelial Cells through the miR-152/VEGFA Axis

Figure 5

VEGFA is the target gene of miR-152, through which H19 can regulate the VEGFA expression. (a) Binding site between the miR-152 and VEGFA was predicated by TargetScan database analysis (up panel). Dual-luciferase analysis of miR-152 to VEGFA.  < 0.01 vs mimics NC. B–C. The expression of VEGFA mRNA and protein were analyzed by qRT-PCR (b) and Western blot (c) in the miR-152 mimics or mimics NC transfected ox-LDL-HAECs;  < 0.01. (d) Pearson analysis of the correlation between miR-152 and VEGFA expression. (e) VEGFA gene expression was detected by qRT-PCR in the si-H19 transfected ox-LDL-HAECs,  < 0.01. (f) Correlation of the expression of H19 and VEGFA was analyzed by Pearson analysis. G-H. VEGFA mRNA and protein expression were detected by qRT-PCR (g) and Western blot (h) in the indicated groups of the ox-LDL-HAECs.  < 0.01 vs siNC + NC inhibitor group, ##  < 0.01 vs si-H19 + NC inhibitor group.

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