Let-7a Targeting TNFAPI3 Promotes Vascular Endothelial Cell Apoptosis of Pediatric Patients with Henoch–Schönlein Purpura via NF-κB Signaling Pathway

Figure 3

Let-7a affects vascular endothelial cell apoptosis by targeting TNFAIP 3. The expressions of TNFAIP 3 in mRNA (a) and protein level (b) were detected by qRT-PCR and western blot, respectively. , , , compared with control. Ns, no significant difference. (c) Target interaction between let-7a and TNFAIP3 was determined by dual-luciferase activity. After cells were transfected with let-7a inhibitor or/and TNFAIP3 siRNA, TNFAIP3 expressions in mRNA, (d) and protein level (e) and cell viability (f) were detected. (g) Cell apoptosis was measured by flow cytometry. (h) The apoptosis-related proteins were detected by western blot. , , . Ns, no significant difference.

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