[Retracted] Role of miR-181b/Notch1 Axis in circ_TNPO1 Promotion of Proliferation and Migration of Atherosclerotic Vascular Smooth Muscle Cells

Figure 6

Overexpression of Notch1 counteracted inhibition effects of proliferation and migration of circ_TNPO1 knockdown in ox-LDL-induced human VSMCs. (a) RT-qPCR assay determined Notch1 mRNA expression level in VSMCs transfected with si-NC, or si-circ_TNPO1, or si-circ_TNPO1 along with pcDNA plasmid or si-circ_TNPO1 along with pcDNA-Notch1 plasmid. (b) The effect of Notch1 overexpression on the proliferative activity of ox-LDL-induced VSMCs transfected with si-circ_TNPO1 was determined by the CCK-8 assay. (c) The effect of Notch1 overexpression on the migrating activity of ox-LDL-induced VSMCs transfected with si-circ_TNPO1 was determined by Transwell assay. (d) The effect of Notch1 overexpression on protein expression of Ki67, PCNA, MMP2, E-cadherin, and N-cadherin in ox-LDL-induced VSMCs transfected with si-circ_TNPO1 was determined by Western blot assay. .

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