The Role of Osthole on TGF-β-Induced Lung Epithelium Apoptosis Injury and Epithelial-Mesenchymal Transition-Mediated Airway Remodeling in Pediatric Asthma

Figure 2

The effect of osthole on TGF-β1-induced epithelial-mesenchymal transition in 16HBE cells. 16HBE cells were treated with TGF-β1 (10 ng/ml) with or without osthole (10 μM) for 24 h. (a) The migrative ability of 16HBE cells determined by transwell assay (n = 5). (b) Changes in cell morphology observed via inverted microscopy (x100). (c) The mRNA levels of epithelial markers (E-cadherin) and mesenchymal marker (N-cadherin and Snail) in 16HBE cells detected by qRT-PCR. (d) Western blot performed to measure the protein levels of E-cadherin, N-cadherin, and Snail in 16HBE cells. (e) The expression of E-cadherin (red) evaluated by immunofluorescence staining, and nucleus was stained with DAPI (blue) (x200). (f) N-cadherin (red) expression evaluated by IF staining, and nucleus was stained with DAPI (blue) (x200). , versus the indicated group.

(a)

(b)

(c)

(d)

(e)

(f)