The Role of Osthole on TGF-β-Induced Lung Epithelium Apoptosis Injury and Epithelial-Mesenchymal Transition-Mediated Airway Remodeling in Pediatric Asthma

Figure 3

Osthole inhibited MAPK and TGF-β/Smad pathways in TGF-β1-induced 16 HBE cells. (a) The total levels and phosphorylated levels of Erk1/2, p38, and JNK of the MAPK signaling pathway determined by Western blot. (b) The phosphorylation levels of Smad2, Smad3, and total levels of Smad2/3 detected by Western blot. (c) The effect of MAPK inhibitor on TGF-β1-induced apoptosis in 16HBE cells detected by flow cytometry, SP600125: JNK inhibitor, SB203580: p38 inhibitor, and PD98059: Erk inhibitor. After transfected with the si-NC vector, or si-Smad2 vector, or si-Smad3 vector for 48 h, 16HBE cells were induced by TGF-β1. (d) Western blot performed to detect the expression of Smad2, Smad3, Snail, E-cadherin, and N-cadherin. (e) Transwell assay carried out to detect cell migration (n = 5). , , , no significance versus the indicated group.

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