[Retracted] The Inflammasome NLRC4 Protects against Cryptococcus gattii by Inducing the Classic Caspase-1 to Activate the Pyroptosis Signal

Figure 1

Internalized Cryptococcus activates the NLRC4 inflammasome, thereby activating caspase-1-related IL-1β and IL-18 secretion. (a) NLRC4 mRNA expression in THP-1 cell-derived macrophages were infected with the yeast form of cryptococcus at the indicated MOI for the indicated time. (b) IL1-β mRNA expression in THP-1 cell-derived macrophages were infected with the yeast form of cryptococcus at the indicated MOI for the indicated time. (c) Different ratios of Cryptococcus were used to stimulate BMDC cells, and IL-1β and IL-18 in the supernatant were determined by ELISA. (d) The THP-1 cell-derived macrophages and MHS cells were stimulated with Cryptococcus for 2 hours, and the expression levels of IL-1β mRNA and IL-18 were measured by real-time PCR. (e) WB was used to detect the activation of NLRC4 protein by Cryptococcus in THP-1 and BMDC cells, respectively. (f) Detection of caspase-1 activation by Cryptococcus in BMDC. (g) Use NLRC4 siRNA and Control siRNA to knock down BMDC, respectively, and detect the expression changes of downstream IL-1β and IL-18 by ELISA.

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