[Retracted] The Inflammasome NLRC4 Protects against Cryptococcus gattii by Inducing the Classic Caspase-1 to Activate the Pyroptosis Signal

Figure 3

NLRC4 is required for Cryptococcus-mediated casepase-1 activation and pyroptosis. Peritoneal macrophages were plated at 5 × 10^5 cells/well and treated withR265, LPS. Cells were washed, lysed, (a) and 100 μg of lysates were assayed using Caspase-1 assay kit (ab39412).PBS was used as a control. (b) ROS was measured by staining the Peritoneal macrophages with DCFDA cellular ROS detection assay kit according to the manufacturer’s instructions. ROS generation was observed under a fluorescence microscope at 200x magnification (left). ROS generation was analyzed by flow cytometry (right). (c) Pyroptosis was assessed by measuring LDH release in the supernatant. (d) Mouse BALF cell number of WT & NLRC4-/-. (e) Peritoneal macrophages from WT and NLRC4-/- mice were incubated with GFP-R265 for 6 hours, and phagocytosis was detected by flow cytometry. 5, , and .

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