[Retracted] Antitumor Activity of lncRNA NBAT-1 via Inhibition of miR-4504 to Target to WWC3 in Oxaliplatin-Resistant Colorectal Carcinoma

Figure 2

NBAT-1 inhibited the OXA-resistant CRC growth. (a) OXA-resistant CRC and parental CRC cells were treated with indicated increasing doses of OXA for 48 h and the cell survival was determined by the CCK8 assay. (b) Expression levels of NBAT-1 were detected by RT-qPCR in the HCT116/OXA and SW480/OXA cells transfected with either empty vector or NBAT-1 expressing vector. (C, D) Cellular proliferation of NBAT-1-transfected HCT116/OXA (c) and SW480/OXA (d) cells was analyzed by the CCK-8 assay. (E, F) Cell cycle was analyzed by FACS flow cytometry in the NBAT-1-transfected and empty vector-transfected HCT116/OXA (e) and SW480/OXA (f) cells. (g) HCT116/OXA and SW480/OXA cells transfected with control lentivirus vector or NBAT-1 lentivirus were implanted into the flanks of nude mice. The NBAT-1 mRNA expression was detected by RT-qPCR in the grown xenograft tumors. (h) Tumor volume were measured at indicated days in the HCT116/OXA and SW480/OXA transfected with NBAT-1 or control xenograft mice. . Each data came from three independent assays.

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