[Retracted] Antitumor Activity of lncRNA NBAT-1 via Inhibition of miR-4504 to Target to WWC3 in Oxaliplatin-Resistant Colorectal Carcinoma

Figure 3

NBAT-1 suppressed miR-4504 in OXA-resistant CRC cells. (a) The predicted binding sequence of NBAT-1 with miR-4504 in miRDB MicroRNA Target Prediction Database (http://mirdb.org/). (b) miR-4504 expression was detected by qRT-PCR in the OXA-resistant and sensitive CRC tissues. (c) miR-4504 expression was detected by qRT-PCR in the OXA-resistant CRC cells. (D, E) Interaction between the miR-4504 and NBAT-1 was analyzed by dual-luciferase reporter assay in the HCT116/OXA (d) or SW480/OXA (e) cells cotransfected with wild-type (WT) or mutant of NBAT-1 and miR-4504 mimics. miR-scramble (miR-NC) was used as negative controls. (f) miR-4504 expression was detected by qRT-PCR in the HCT116/OXA and SW480/OXA OXA-resistant CRC cells transfected with NBAT-1 or empty vector. (G, H) Cellular proliferation was assayed by CCK8 assay in the HCT116/OXA (g) and SW480/OXA (h) cells. . Each data came from three independent assays.

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