[Retracted] An Association between EMX2 Variations and Mayer-Rokitansky-Küster-Hauser Syndrome: A Case-Control Study of Chinese Women

Table 1

Primers and PCR conditions for EMX2 gene amplification.

Primer	Primer sequence (5′-3′)	PCR conditions
Exon1F	CGCTAGGCTAGAGGAATCTGTCTGT	3 min at 97°C; 30 s at 97°C, 30s at 68°C (−0.5 °C per cycle), 90 s at 72°C (30 cycles), and 3 min at 97°C; ‘30 s at 97°C, 30 s at 61°C, 90 s at 72°C (5 cycles), and 10 min at 72°C
Exon1R	CAACTTCTCCGTTCGCACCC
Exon2F	GAGCAGGCGTTCCCTTCGT	3 min at 94°C; 1 min at 94°C, 1 min at 63.4°C, 1 min at 72°C (35 cycles), and 10 min at 72°C
Exon2R	GGGCAGATTGGCACTTACAGC
Exon3F	GCTGGGTCTTTGCTGAGTC	3 min at 94°C; 1 min at 94°C, 45s at 67.3°C, 2 min at 72°C (35 cycles), and 10 min at 72°C
Exon3R	TGAGGAGCCTGGGTTTCTT