4-Methoxydalbergione Elicits Anticancer Effects by Upregulation of GADD45G in Human Liver Cancer Cells

Figure 7

4MOD exerts anticancer effects by upregulating GADD45G expression in HCC. (a) The relative GADD45G mRNA expression was detected by qRT-PCR in shGADD45G-transfected cells. (b, c) SK-HEP-1 and HuH-7 cells were transfected with negative control (shNC) or shGADD45G, respectively. CCK8 was used to detect cell viability after treatment with or without 4MOD (20 μM) for 48 h. (d) SK-HEP-1 was transfected with shNC or shGADD45G, respectively. Wound healing assay was used to detect cell migration after treatment with or without 4MOD (20 μM) for 48 h. (e) HuH-7 was transfected with shNC or shGADD45G, respectively. Wound healing assay was used to detect cell migration after treatment with or without 4MOD (10 μM) for 48 h. (f) SK-HEP-1 were transfected with shNC or shGADD45G, respectively. Flow cytometry was used to detect cell apoptosis after treatment with or without 4MOD (40 μM) for 48 h. (g) HuH-7 was transfected with shNC or shGADD45G, respectively. Flow cytometry was used to detect cell apoptosis after treatment with or without 4MOD (20 μM) for 48 h. , .

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