Expansion and Activation Kinetics of Immune Cells during Early Phase of GVHD in Mouse Model Based on Chemotherapy Conditioning
Figure 3
Dendritic cells chimerism and activation pattern in GVHD developing mice. Spleens of allogeneic transplanted recipients were evaluated for dendritic cell chimerism and maturation marker. (a, b) Frequency and expansion pattern of donor (CD11c+ and H-2Kb) versus host (CD11c+ and H-2Kd) dendritic cells in the spleen of recipient mice in GVHD developing setting. (c) Maturation status of DCs was evaluated using expression level of MHC class II on DCs surface. Three days after BMT donor DCs has higher expansion with more expression of maturation marker on cell surface. (◆): donor DCs (CD11c+ and H-2Kb), (◊): host DCs (CD11c+ and H-2Kd). All values are mean ± SE for 4 to 6 animals in each group per time point. Differences were analyzed statistically employing U-test, compared between groups ().
