Expansion and Activation Kinetics of Immune Cells during Early Phase of GVHD in Mouse Model Based on Chemotherapy Conditioning

Table 1

Effect of conditioning on the different immune cell of bone marrow and spleen. Female BALB/c mice were treated with busulfan (80 mg/kg) followed by cyclophosphamide (200 mg/kg). Bone marrow and spleen cellularity plus immune cell phenotype were evaluated using flowcytometry before and after conditioning. Total and individual immune cells in the bone marrow are more sensitive to the conditioning compare to the spleen cells. ¹CD3+CD4+, ²CD3+CD8+, ³CD44^lowCD62^high, ⁴CD44^highCD62^low, ⁵CD19+, ⁶DX5+, ⁷CD11c+, and ⁸CD11b+. (^∗𝑃<.05)

Time point	Organ	Cellularity	T helper^1	T cytotoxic^2	Naïve T cells^3	Effector memoryT cells^4	B cell^5	NK cell^6	DCs^7	Myeloid; lineage^8
Before; Conditioning; (D-7)	BM	39±0.5	0.34±0.02	0.11±0.02	0.05±0.01	0.16±0.01	9±0.6	0.6±0.06	0.44±0.04	15±0.32
After; Conditioning; (D0)	BM	2±0.2	0.1±0.01	0.08±0.01	0.04±0.01	0.06±0.01	0.13±0.06	0.05±0.01	0.03±0.004	0.2±0.05
Decrement; (%)		95%∗	71%∗	27%	20%	63%∗	99%∗	92%∗	93%∗	99%∗
Before; Conditioning; (D-7)	SP	189±5.6	39±1.7	14±1.5	23±2.1	14±1.1	69±3.5	10.4±1	5±0.3	—
After; Conditioning; (D0)	SP	70±2.6	26±0.9	12±0.9	19±1.3	7±0.4	16±2.02	1.4±0.2	1±0.1	—
Decrement; (%)		63%∗	33%∗	14%	17%	50%∗	77%∗	87%∗	80%∗	—

^∗𝑃-value <.01.