Serum and Salivary IgE, IgA, and Antibodies to Dermatophagoides pteronyssinus and Its Major Allergens, Der p1 and Der p2, in Allergic and Nonallergic Children
Figure 2
Representative reference curves of IgA antibodies to Dermatophagoides pteronyssinus (Dpt) (a) and its major allergens Der p1 (b) and Der p2 (c) in saliva samples. A pool of saliva samples with high levels of specific IgA antibodies for each allergen and established as containing 1000 arbitrary units per milliliter (AU/mL) was used for each reference curve, using serial double dilutions ranging from 1000 to 0.977 AU/mL. The curves were constructed with the GraphPad Prism 5.0 software by plotting the absorbance (OD) and concentration (AU/mL) values, using the quadratic curve fit. The correlation coefficients () are indicated and the detection limits for these assays were 7.8 AU/mL for IgA anti-Dpt, and 3.9 AU/mL for IgA anti-Der p1 and IgA anti-Der p2.
