Growth of Human Colorectal Cancer SW1116 Cells Is Inhibited by Cytokine-Induced Killer Cells
Figure 2
Cytotoxicity of CIK cells in vitro. (a) Cytotoxicity of PBMCs against SW1116 cells. (b) Cytotoxicity of CIK cells against SW1116 cells. (c) Cytotoxicity of CIK cells against U251 cells. At an effector : target ratio of 100 : 1, the percentage lysis of SW1116 cells after the addition of PBMCs was 9%. At effector : target ratios of 1 : 1, 5 : 1, 10 : 1, 20 : 1, and 50 : 1, the percentage lysis after the addition of CIK cells was 3%, 23%, 42%, 62%, and 68%, respectively, for SW1116 cells and 2%, 13%, 32%, 48%, and 54%, respectively, for U251 cells. (d) Observation of the distribution of CIK cells by HE staining, ×100 and ×200. CIK cells adsorbed to and aggregated around the SW1116 cells. For cytotoxic assay, each experiment was performed in triplicate and was repeated at least three times, and the results are expressed as mean ± SD. ()
