Hepatitis C Virus Induces Regulatory T Cells by Naturally Occurring Viral Variants to Suppress T Cell Responses
Figure 8
Tetramer depletion of T cells specific for variant peptides restores T cell proliferative response to cognate NS3358–375. (a–c) Variant tetramer depleted listed at the top of the panels. Tetramer depletion was done using PBMC from subject PB3019. PBMC incubated with variant peptide for 3 hrs and then stimulated with WT peptide for 7 days (gray, stripped, and black panels). Black histogram is indicative of add-back experiment, in which, 10% represents approximately the same number of cells as normal mix population. Each experiment was done in triplicate, replicated 3 times for a total 9 data points. (d) HLA-DR15 CLIP tetramer-depleted cells shown in yellow compared to PBMC from nondepleted PBMC proliferation assay at 7 day, each experiment was done in triplicate ().
