Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance

Figure 4

Effects of the treatments with OVA on the frequency of CD25⁺ cells in the small intestine. Freshly isolated IELs from BALB/c (a) and DO11.10 (b) mice were gated for CD3⁺/CD4⁺, CD3⁺/CD8α ⁺, and CD3⁺/CD8β ⁺ cells and analyzed for expression of CD25 by flow cytometry. Blank histograms indicate isotype control staining. CD3⁺/CD8β ⁺ subset illustrated at the right column is part of the CD3⁺/CD8α ⁺ population. (c) Data represent mean ± SEM (𝑁=5), in three independent experiments. An increased frequency of CD25⁺ cells can be observed only in CD8β subset of IELs from DO11.10 mice treated with OVA by oral route.

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