DNA Methyltransferase 3B Gene Promoter and Interleukin-1 Receptor Antagonist Polymorphisms in Childhood Immune Thrombocytopenia

Table 1

Allele types, PCR conditions, and PCR primers designed to amplify fragments harboring the polymorphic sites.

	DNMT3B promoter	IL-1 Ra
Type of polymorphism	Single-base C/T	86-bp VNTR
Site of polymorphism	Position 149	Intron 2
PCR primers
Upstream	5′-TGCTGTGACAGGCAGAGCAG-3′	5′-CTCAGCAACACTCCTAT-3′
Downstream	5′-GGTAGCCGGGAACTCCACGG-3′	5′-TCCTGGTCTGCAGGTAA-3′
Digestion	AvrII	—
Allele size (bp)	C: 380	I: 410
		II: 240
	T: 207+173	III: 325
		IV: 500