Host Susceptibility to Brucella abortus Infection Is More Pronounced in IFN-γ knockout than IL-12/β2-Microglobulin Double-Deficient Mice
Figure 3
IFN-γ, TNF-α, and NO production induced by B. abortus in IFN-γ KO or IL-12/β2-m KO splenocytes. Spleens cells (1 × 106 cells) were stimulated with B. abortus S2308 (MOI 100 : 1), Con A (5 μg/mL), or E. coli LPS (1 μg/mL). Levels of TNF-α (a) and IFN-γ (b) were measured by ELISA after 48 and 72 hrs, respectively. Levels of (c) were measured by Griess reaction after 48 hrs of antigen stimulation. Statistically significant differences in relation to C57BL/6 mice are indicated by an asterisk (), in relation to IL-12/β2-m−/− by two asterisks, and those between unstimulated and stimulated spleen cells are indicated by the symbol # ().
