Both and Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults
Figure 1
Effect of blocking anti-MHC class I and anti-MHC class II antibodies on the FHA-specific IFN-γ production. Isolated PBMC were stimulated in vitro for 72 h with 1 μg/mL FHA in the presence or absence of blocking anti-MHC-II (a) and (b) or anti-MHC-I (c) and (d) antibodies or in the presence or absence of control isotypes. Unstimulated cells were tested as control. IFN-γ concentrations were measured in the cell culture supernatants and values obtained for unstimulated cells were subtracted from those obtained for stimulated cells. Circles represent the individual IFN-γ concentrations obtained after stimulation with FHA in the presence of anti-MHC antibodies or control isotypes. Results obtained for the same samples are linked. *; **; ***.
