Both and Lymphocytes Participate in the IFN-γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults
Figure 4
Effect of the CD4+ or CD8+ depletion on the FHA-induced IFN-γ production by PBMC from B. pertussis-infected or vaccinated children. Isolated PBMC were stimulated in vitro for 72 h with 1 μg/mL FHA after CD4+ (a) or CD8+ (b) depletion. Results from infected children are represented by columns 1 to 5 whereas those from vaccinated children are indicated by numbers 6 to 16. Open columns represent the values obtained for the nondepleted cell suspension and grey columns those obtained after CD4+ or CD8+ depletions. The arrows indicate the samples characterized by a higher IFN-γ secretion in CD8-depleted cell suspension compared to the PBMC.
