NFAT Signaling in Osteoblasts Regulates the Hematopoietic Niche in the Bone Microenvironment

Figure 3

Lin^-Sca1⁺cKit⁺ (LSK) hematopoietic stem/progenitor cells are increased in dnNFAT^OB mice. Bone marrow was flushed from tibiae and femora of 12-week-old control and dnNFAT^OB mice and analyzed by flow cytometry for detection of LSK cells. (a) Representative dot plots from control and dnNFAT^OB mice showing c-Kit⁺Sca-1⁺ cells gated from the Lin^- cell population. (b) Percentages of LSK cells were analyzed from control () and dnNFAT^OB () mice. Mean indicated by black bar; * when compared to the control.

(a)

(b)