Lymphoid Progenitor Cells from Childhood Acute Lymphoblastic Leukemia Are Functionally Deficient and Express High Levels of the Transcriptional Repressor Gfi-1
Figure 1
Hematopoietic and progenitor cell frequencies are critically reduced in acute lymphoblastic leukemia (ALL). The primitive cell fraction was identified in control and ALL bone marrow (BM) on the basis of its functional expression of aldehyde dehydrogenase. Two ALL samples and one control are shown (a). Mononuclear cells from ALL BM were resolved in stem and lymphoid progenitor populations according to CD34, CD45RA, CD7, and CD10 expression, and their frequencies were calculated and compared to normal counterparts (b). HSC were identified as Lin−CD34+CD45RA− and MPP as Lin−CD34+CD45RA+. MPP subfractionation gave rise to TP (Lin−CD34+CD45RA+CD7+CD10−), ELP (Lin−CD34+CD45RA+CD7+CD10+), and CLP (Lin−CD34+CD45RA+CD7−CD10+). HSC: hematopoietic stem cells; MPP: multipotent and multilymphoid progenitor cell fraction; TP: T-cell progenitors; ELP: early lymphoid progenitors; CLP: common lymphoid progenitors.
