Anti-CD3 Antibody Treatment Induces Hypoglycemia and Super Tolerance to Glucose Challenge in Mice through Enhancing Glucose Consumption by Activated Lymphocytes
Figure 8
Effect of anti-CD3 antibody treatment on T and B cell activation in vitro and in vivo. (a) In vitro experiment. Spleen cells 1 × 106/well in 24-well plate were stimulated with anti-CD3 antibodies (3 g/mL) or Isotype IgG (3 g/mL) for 24 hours. Thereafter, The cells were harvested and stained with CD4, CD8, B220, and CD69 and analyzed by flow cytometry. (b) In vivo experiment. C57BL/6 mice were treated with intraperitoneal injection of anti-CD3 antibodies (50 g/mouse) or Isotype IgG (50 g/mouse). Three mice were included in each group. Twenty-four hours later, all mice were killed, and spleen cells of each individual mouse were prepared and stained for CD4, CD8, B220, and CD69. The expression of these markers was examined by flow cytometry.
