Tumor-Activated T Cells from Gastric Cancer Patients Induce the Antitumor Immune Response of T Cells via Their Antigen-Presenting Cell-Like Effects
Figure 1
APC-like characteristics of tumor-activated γ δ T cells. (a) The proliferation of γ δ T cells was analyzed from [3H] thymine deoxyriboside (TdR) incorporation. Irradiated (30 Gy) PBMCs or tumor tissue cells (2 × 104 cells/well) were cocultured with peripheral-derived γ δ T cells (6 × 104 cells/well) from patients with gastric cancer in 96-well plates for 3 days in the presence of IL-2 (200 U/mL) and IL-15 (20 ng/mL). [3H]TdR incorporation was measured during the last 12 h of the incubation. Results are expressed as means ± SD (cpm) of three wells. The data are representative of at least nine independent experiments. (b) FCM was used to analyze gating for the CD3+TCRγ δ+ cell population; phenotypes including CD80, CD83, CD86, and HLA-DR on the γ δ T cells from PBMCs of healthy donors (control), peripheral-derived, tumor tissue-derived, and tumor-activated γ δ T cells. Results are representative of nine independent experiments. (c) Uptake and sorting of soluble proteins in tumor-activated γ δ T cells. γ δ T cells were cocultured with CFSE-prelabeled tumor cells (green fluorescence) for the indicated time. The cells were then collected, cytocentrifuged, and stained with TCRγ δ-PECY5 mAb (red fluorescence). The interaction between γ δ T cells and tumor cells was observed using confocal microscopy. The arrow shows the colocalization of the TCR of γ δ T cells with some components of tumor cells. Results are representative of three independent experiments. (d) Tumor-activated or peripheral-derived γ δ T cells (1 × 106 cells/mL) were cultured in RPMI 1640 containing IL-2 (200 U/mL) and IL-15 (20 ng/mL) for 12 h, and the culture supernatants were collected and assayed for IFN-γ, IL-1, IL-4, IL-6, IL-10, and IL-12 using ELISA. Data are representative of five independent experiments. *.
