Inhibition of 5-Lipoxygenase Pathway Attenuates Acute Liver Failure by Inhibiting Macrophage Activation
Figure 5
AA-861 significantly abrogated the increased number of ED1-positive cells in liver and increased level of TNF-α in serum from D-GalN/LPS-treated rats. (a) Representative photomicrographs (400x) of liver sections immunostained with ED1 antibody from control (upper left panel) and 8-hr D-GalN/LPS-treated rats receiving placebo (middle panel) or AA-861 (right panel). A representative number of ED1-positive cells (Kupffer cells) are denoted by arrows. In D-GalN/LPS-treated rats, ED1-positive cells were located mainly in the midzonal area surrounding the damaged perivenular area. (b) The number of ED1-positive cells per HPF in liver tissue sections from control (left bar) and D-GalN/LPS-treated animals receiving placebo (middle bar) or AA-861 (right bar) for 8 hr. (c) The level of TNF-α in serum from control (left bar) and D-GalN/LPS-treated animals receiving placebo (middle bar) or AA-861 (right bar) for 8 hr. Results are expressed as mean ± standard error.
