fMLP-Induced IL-8 Release Is Dependent on NADPH Oxidase in Human Neutrophils

Figure 5

NADPH oxidase inhibition interferes with intracellular pH changes. BCECF-AM-loaded neutrophils were incubated with vehicle (a), 500 μM HMAP (b), 10 μM DPI (c), or 500 μM amiloride (d) for 10 min. A basal level was measured before 100 nM fMLP was added, and the intracellular pH was recorded for 500 s.

(a)

(b)

(c)

(d)