Human Myeloma Cell Lines Induce Osteoblast Downregulation of CD99 Which Is Involved in Osteoblast Formation and Activity

Figure 5

CD99 agonist monoclonal antibody increases RUNX2 and JUND expression in normal human osteoblasts (hOBs). hOBs, treated with anti-CD99 agonist monoclonal antibody or with mouse IgG for 0, 4 and 6 hours (h), were lysed and analyzed by western blot analysis to detect the protein levels of RUNX2 (upper panel) and JUND (lower panel) OB trascription factors. The histograms represent the mean optical density (OD) of RUNX2 or JUND ratio normalized to the OD of -Actin. Data are presented as mean ± SE. The figure shows one representative of three independent experiments.