Enhanced Cysteinyl-Leukotriene Type 1 Receptor Expression in T Cells from House Dust Mite-Allergic Individuals following Stimulation with Der p

Figure 1

Der p effect on T cell CysLT₁ and CysLT₂ mRNA and protein expression. Comparison of CysLT₁ and CysLT₂ mRNA expression in CD4⁺ and CD8⁺ T cells from healthy controls and HDM-allergic (HDMA) patients following stimulation with the Der p allergen (200 AU/mL). PBMC from healthy control and HDMA subjects were cultured for 48 h (qPCR) or 72 h (FACS) in the presence of glycerol vehicle or Der p before CD4⁺ and CD8⁺ T cells were purified and collected for analysis. CysLT₁ (a) and CysLT₂ (b) mRNA expression was measured by real-time quantitative PCR analysis. Data are presented as fold (ΔΔCt) increases over GAPDH mRNA (±SEM). and , relative to vehicle glycerol; for controls; for HDMA. Cell surface expression of CysLT₁ (c) receptor was evaluated using rabbit polyclonal anti-CysLT₁ receptor Ab, followed by labeling with FITC-conjugated goat anti-rabbit IgG. Cells were further incubated with anti-CD4 PE-Cy5 and anti-CD8 PE Ab before analysis on a FACSCalibur flow cytometer. Data are expressed as geometric mean (±SEM) fluorescence intensity (MFI). ; for controls; for HDMA.

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