RNA 1 and RNA 2 Genomic Segments of Chronic Bee Paralysis Virus Are Infectious and Induce Chronic Bee Paralysis Disease

Figure 6

Antigenomic RNA detection by specific RT-PCR. 1.2% agarose gel electrophoresis of the PCR products (amplicons). (a) Three samples of negative control (NC) and three samples of bees inoculated with CBPV particles (VP), five samples of bees inoculated with CBPV naked RNAs, and eight samples of bees inoculated with CBPV major RNAs. (b) Three samples of negative control (NC), four samples of bees inoculated with inoculation buffer (IB), and one sample of bees inoculated with viral particle (VP) were tested. M: 100 bp DNA marker (TrackIt, Invitrogen).

(a)

(b)