Cloning of Interleukin-10 from African Clawed Frog (Xenopus tropicalis), with the Finding of IL-19/20 Homologue in the IL-10 Locus

Table 1

Primers used in the study.

Primer	Sequence (from 5′ to 3′)	Application
UPM	CTAATACGACTCACTATAGGGC	RACE-PCR
IL10-5Rout	CATCTCCGCTTTGACATTTCACCGT	5′-RACE
IL10-5Rin	CCGTTTTGCATGTGAAGAAGA	5′-RACE
IL10-3Fout	AGCAAGGTATCTACAAGGCAATGGG	3′-RACE
IL10-3Fin	ATGGGAGAATTCGATATTTTGATTG	3′-RACE
xeIL-10F1	TGGAAATTGTCTTACTTCAA	Real-time PCR
xeIL-10R1	TGTTTAATTTGCTTGATAGC	Real-time PCR
-Actin-F	GGTCGCCCAAGACATCAG	Real-time PCR
-Actin-R	GCATACAGGGACAACACA	Real-time PCR