Branched Polyethylenimine-Superparamagnetic Iron Oxide Nanoparticles (bPEI-SPIONs) Improve the Immunogenicity of Tumor Antigens and Enhance Th1 Polarization of Dendritic Cells

Figure 4

Characterization of DCs loaded with U266 cells. DCs were analyzed based on (a) flow cytometry for phenotype, (b) uptake of tumor antigens (PKH67⁺ CD11c⁺), and (c) migration capacity in response to CCL21. There were no significant differences in these parameters among the groups. (d) Cytokine production by DCs after 24-h stimulation with CD40L-transfected J558 cells was measured using ELISA. The 2 h postirradiated DCs and bPEI-SPION 2 h postirradiated DCs showed significantly higher levels of IL-12p70 secretion compared with 16 h postirradiated DCs. Data are representative of four independent experiments ().

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