Branched Polyethylenimine-Superparamagnetic Iron Oxide Nanoparticles (bPEI-SPIONs) Improve the Immunogenicity of Tumor Antigens and Enhance Th1 Polarization of Dendritic Cells

Figure 5

T cell polarization of DCs. Intracellular IFN-γ and IL-4 levels were measured using flow cytometry. bPEI-SPIONs 2 h postirradiated DCs exhibited the highest Th1 response (IL4⁻IFNγ⁺) up to 18% of total T cells. (b) Amount of secreted IFN-γ and IL-4 was measured by ELISA. T cells polarized by bPEI-SPION 2 h postirradiated DCs presented a significant increase level of IFN-γ. Data are representative of three independent experiments ().

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