Respiratory Syncytial Virus Nonstructural Proteins Upregulate SOCS1 and SOCS3 in the Different Manner from Endogenous IFN Signaling

Figure 3

Expression of RSV NS proteins resulted in impaired IFN-α-induced STAT phosphorylation. A549 cells were incubated with or without IFN-α (5000 U/mL) for 30 min and then transfected with empty vectors (a), pNS1 (b), or pNS2 (c) for the indicated times. Total protein lysates were subjected to Western blot analysis using the following antibodies: anti-pSTAT1 (1 : 1000), anti-STAT1 (1 : 1000), anti-pSTAT2 (1 : 1000), or anti-STAT2 (1 : 1000). The results shown are representative of three independent experiments. The data were analyzed by densitometry, normalized to β-actin protein levels, and are shown as the mean ± SE. versus untreated controls.

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