Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology

Figure 2

Immunofluorescence patterns on Crithidia luciliae revealing the absence or presence of antibodies against dsDNA. Samples are determined as anti-dsDNA negative if the kinetoplasts of the cells do not fluoresce, irrespective of fluorescent nuclei (a), basal bodies (b), or both (c). Samples are determined as anti-dsDNA positive as soon as the kinetoplasts reveal fluorescence signals above a given threshold (see arrows) which may be accompanied by additional fluorescence of the nuclei (d), the basal body (e), or both (f).