The Human IL-22 Receptor Is Regulated through the Action of the Novel E3 Ligase Subunit FBXW12, Which Functions as an Epithelial Growth Suppressor

<div>FBXW12 is functional as an E3 ligase subunit to ubiquitinate IL-22R. (a) FBXW12-Myc plasmid was transfected (3 <i>μ</i>g) into HeLa cells and lysates immunoprecipitated with Myc antibody or IgG control and immunoblotted for Myc (left panel) and IL-22R (right panel). (b) Immunopurified FBXW12-Myc was probed for association with the SCF constituent Skp-1. (c)<i> In vitro</i> ubiquitination assay: IL-22R by itself or with needed cofactors of the ubiquitination reaction (E1 + E2 enzymes and/or Skp-1 and cullin-1) was incubated for 1 h before IL-22R immunoblotting; the presence of the upper band in lane 2 indicates IL-22R ubiquitination. Data are representative of two experiments performed on different days.</div>

Journal of Immunology Research

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Figure 2

Figure 2: The Human IL-22 Receptor Is Regulated through the Action of the Novel E3 Ligase Subunit FBXW12, Which Functions as an Epithelial Growth Suppressor 

Figure 2 | The Human IL-22 Receptor Is Regulated through the Action of the Novel E3 Ligase Subunit FBXW12, Which Functions as an Epithelial Growth Suppressor 