The Human IL-22 Receptor Is Regulated through the Action of the Novel E3 Ligase Subunit FBXW12, Which Functions as an Epithelial Growth Suppressor

Figure 5

FBXW12 depletion increases cell cycle progression. HeLa cells were transfected with empty vector or FBXW12 shRNA containing a GFP reporter and treated for 8 h with IL-22 or vehicle before DNA staining with Vybrant Dyecycle Ruby for cell cycle analysis. (a) GFP-Hi cells containing FBXW12 shRNA were gated and compared with nontransfected cells. (b) Histograms of cell DNA content were used to analyze cell cycle phase. (c) Proportion of cells with progression out of phase G1 for the indicated groups is shown ( by ANOVA on a single experiment with 4 replicates; data are representative of 4 experiments performed on different days).

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