Storage Conditions of Conjugated Reagents Can Impact Results of Immunogenicity Assays

Figure 3

ECL signal in relative light units (RLU) of the system suitability controls: negative control (NQC: pooled serum) and low and high positive controls (LQC: 15 ng/mL, HQC: 1000 ng/mL of affinity-purified anti-idiotype goat polyclonal antibody in NQC) analyzed with reagents stored in PBS and HSB subjected to multiple freeze-thaw (F/T) cycles. Error bars show mean with standard deviation.