TLR Stimulation Dynamically Regulates Heme and Iron Export Gene Expression in Macrophages

Figure 4

Macrophages downregulate Flvcr and Fpn1 expression in response to M1 and M2 polarization. Primary macrophages (M0) were treated with LPS/IFNγ or IL4 to polarize to M1 or M2 state, respectively. Both M1- and M2-polarized macrophages downregulated Fpn1 to a similar extent as compared to M0 macrophages. Interestingly, Flvcr downregulation in M2 macrophages occurred later and was less pronounced than in M1 macrophages. The mean and SEM are shown ( mice). Below each graph is a table summarizing statistical significance for all pairwise comparisons at each time point. indicates . The data is representative of 2 independent experiments.