TLR Stimulation Dynamically Regulates Heme and Iron Export Gene Expression in Macrophages

Figure 5

Macrophages downregulate Flvcr expression in response to multiple TLR agonists, and the downregulation is TLR-dependent. Primary BMDM were generated from control C57BL/6 or Tlr4^−/− and Ly96^−/− mice and stimulated with various TLR agonists and IFNγ. Flvcr and Fpn1 downregulation in response to LPS was abrogated in Tlr4^−/− and Ly96^−/− macrophages. Stimulation with TLR1/2 agonist Pam3Csk4 (Pam3) and the TLR3 agonist polyinosinic:polycytidylic acid (pIC) also caused a decrease in Flvcr and Fpn1 expression in both wild-type and Tlr4^−/−; Ly96^−/− macrophages. The mean and SEM are shown ( mice). ND: not detected, as amplification was below the lower limit of detection. The data are representative of 2 independent experiments. . .