Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance

Figure 2

Assay formats and platforms. For each platform, a bridging format was used with the biotinylated drug (Novimab) as the capture antibody. The drug (Novimab) was also used as the detection antibody and was conjugated to a specific labeling molecule appropriate to each of the different platforms. Only the ELISA assay was developed using a tertiary antibody directed against the detection antibody TAG. HRP: Horseradish Peroxidase, sTAG: SULFO-TAG, and AF: Alexa Fluor 647.