Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance
Table 1
Sample dilution factors according to the different methods (platforms and additional steps).
Technology used
ELISA
MSD
Gyrolab
AlphaLISA
Acid dissociation step
No
Yes
No
Yes
No
Yes
No
Yes
Target depletion step
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
No
Yes
Dilution induced by the target depletion step
1 in 3
1 in 3
1 in 3
1 in 3
1 in 3
1 in 3
1 in 3
1 in 3
Dilution induced by the acidification step
NA
1 in 5
NA
1 in 5
NA
1 in 2
NA
1 in 5
Dilution induced by the neutralization step/Master Mix addition
1 in 2
1 in 3
1 in 2
1 in 3
1 in 2
1 in 1.5
1 in 5
1 in 3
Final dilution applied before platform analysis
1 in 6
1 in 45
1 in 6
1 in 45
1 in 6
1 in 9
1 in 15
1 in 45
Samples not target depleted were diluted at 1 in 3 in PBS-1% BSA 0.05% tween.
