The Generation and Characterization of Recombinant Protein and Antibodies of Clostridium perfringens Beta2 Toxin

Figure 2

The cytotoxicity of rCPB2 toxin in human colon NCM460 cells. The cytotoxicity of purified rCPB2 protein was ascertained by determining its concentration causing 50% cell death (cytotoxicity 50, CT50) in a given time point in terms of an MTT assay. (a) A representative image (×400) of control NCM460 showed normal morphology of cells. (b) A representative image of NCM460 cells treated with rCPB2 at 20 μg/mL for 12 h exhibited a morphological change of cell death. (c) A representative image of NCM460 was treated for 12 h with supernatant of C. perfringens C59-44 strain. (d) The viability of NCM460 cells treated with indicated concentrations of rCPB2 for indicated times determined by an MTT assay. The data was expressed as the mean ± SD of fold changes of viable cells against the control untreated cells from three triplicated independent experiments. Bars in (a) and (b): 50 μm.

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