Characterization of Interleukin-15-Transpresenting Dendritic Cells for Clinical Use

Figure 3

T-cell stimulatory capacity of IL-15 designer DC in an allo-MLR. Bar graphs depict proliferation of PBL upon 5-day coculture with mock EP DC (white bar), IL-15 EP DC (grey bar), or IL-15/IL-15Rα EP DC (black bar) at a 10 : 1 T-cell/DC ratio. Cocultures were analyzed for CD4⁺ T-cell and CD8⁺ T-cell proliferation within the viable CD3⁺ T-cell population by flow cytometry. Data are shown as mean (±SEM) for 4 independent donors. ; , repeated measures one-way ANOVA with Bonferroni post hoc test. ADC, dendritic cells; IL, interleukin; IL-15Rα, interleukin-15 receptor alpha; ns, not significant; PBL, peripheral blood lymphocytes; SEM, standard error of the mean.