Research Article

Beneficial Effects of Prebiotic Saccharomyces cerevisiae Mannan on Allergic Asthma Mouse Models

Figure 6

Accelerated large airway smooth muscle remodeling in a huASM-MRC2 transgenic allergic asthma mouse model. (a) Schematic of the construct used to generate huASM-MRC2 transgenic (Tg+) mice. The resulting transgenic founders were confirmed by Southern blot, probing Hind III-digested DNA with a 1 kb fragment of the huASM-MRC2 gene (hybridizing to a ~4.3 kb DNA fragment), and PCR analysis using huASM-MRC2 oligonucleotide primers (600 bp product). (b) Mice were OVA-sensitized and OVA-challenged. Control mice received OVA sensitization and PBS challenge. Histology was performed on day 38, 45, or 52. Results are mean ± SD (). . . (c) Thoracic sRaw in allergic huASM-MRC2 transgenic mice (N10, backcrossed onto BALB/c). A shift of the curve to the left indicates Mch sensitivity (AHR). Numerous breaths were rejected from the automated data acquisition due to shallow labored breathing at Mch 25 mg/ml. Data are mean ± SD ().
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