Genetics and Molecular Biology of Epstein-Barr Virus-Encoded BART MicroRNA: A Paradigm for Viral Modulation of Host Immune Response Genes and Genome Stability

Figure 3

Detection of host immune response transcription factors including AP-1 binding fos/jun induced by cotransfection of cell cycle inducer MEKK1 and viral ZEBRA switch protein are shown in three EBV positive lymphoblastoid cell lines. The promoter region of BALF-2, a 5′ region of the BamHI A fragment adjacent to the BALF-2 mRNA start site, has been cloned from B95-8 viral DNA into a firefly luciferase expression vector (PGL-3 (Promega), reporter vector denoted BAP52/32 BLUC), and sequences have been shown to be identical to those published for B95-8. Results of transient transfection of the putative BALF-2 minimal promoter into 3 EBV positive B lymphoblastoid cell lines (Akata, Namalwa, and Raji) are shown (see text).