Secreted Interferon-Inducible Factors Restrict Hepatitis B and C Virus Entry In Vitro

Figure 4

IFN-α induced products compete with HBV for binding to heparin column. (a) Heparin columns were washed with PBS and then applied with differentiated HepaRG cell culture medium (mock) or IFN-α treated differentiated HepaRG cell culture medium (ISG+) or the entry inhibitor Pep19-2.5. Then columns were applied with PBS containing HBV. After washing, HBV was eluted by elution buffer. (b) HBV amount from different elutions were analyzed by HBV-DNA qPCR.

(a)

(b)