Adoptive Cell Therapy of Induced Regulatory T Cells Expanded by Tolerogenic Dendritic Cells on Murine Autoimmune Arthritis

Figure 1

Characteristic profile of tolerogenic DCs derived from D1 mice.iDCs, mDCs, tDCs, and mtDCs were induced to differentiate in vitro, as described in the Materials and Methods, after which CD11c⁺ cells were harvested. (a) These four types of DC were stained with CD86, CD80, IA-IE (thick lines), or isotype-matched mAbs (thin lines), and the expression of these markers was analyzed by FACS using flow cytometry. The frequency of positively stained cells and MFI of a representative of 10 independent experiments are shown in the FACS profile. The mean fluorescence intensity is reported as the mean ± SEM (). for the comparison of mDCs with mtDCs using unpaired t-tests. (b) IL-12 p40, IL-10, and TGF-β expression in DCs was determined by real-time PCR. All results were normalized to the expression of the housekeeping gene β-actin and are expressed as the means ± SEM of six independent experiments. and for the comparisons of the indicated groups using the unpaired t-test.

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