Research Article
Analysis of Sera of Recipients with Allograft Rejection Indicates That Keratin 1 Is the Target of Anti-Endothelial Antibodies
Figure 1
Isolation and identification of AECAs from posttransplant sera. (a) Sera samples from five patients were analyzed by flow cytometry for crossmatches using HUVECs. Normal human serum was used as negative control; HLA-sensitized pooled sera were used as positive control. Profiles before platelet absorption and after platelet absorption are shown. (b) Sample S5 was analyzed by flow cytometry in the presence of HUVEC and cord blood T lymphocytes. The negative control was normal human serum (NC), and the positive control was HLA-sensitized pooled sera.
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