EGFR-Targeted Immunotoxin Exerts Antitumor Effects on Esophageal Cancers by Increasing ROS Accumulation and Inducing Apoptosis via Inhibition of the Nrf2-Keap1 Pathway

Figure 5

PT treatment resulted in caspase-3-dependent apoptosis in KYSE-450 cells. (a) Induction of apoptosis in KYSE-450 cells with medium alone (control), PT (10 nM), or Pan (10 nM) treatment for 16 h. Apoptosis proportion was measured by flow cytometry. (b) Statistical analysis of the percentage of the apoptotic cells. Data was shown with mean ± SD. . (c) Apoptosis-related protein (PARP, cleaved caspase-3, BcL-XL, or Bcl-2) was examined in KYSE-450 cells when treated with medium alone (−), Pan (10 nM), or PT (10 nM) for 16 h.

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